Challenges and Limitations of Human-Derived Cell Lines

Despite their advantages, human-derived cell lines present certain challenges. Issues such as genetic instability, limited lifespan, and variability between donors can affect experimental consistency. Additionally, the complexity of human tissues is difficult to fully replicate in vitro, potentially limiting the applicability of findings. Ensuring the quality and reproducibility of these models requires rigorous standardization and validation protocols. Addressing these limitations is crucial for maximizing the potential of human-derived cell lines in research and therapeutic development.